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First-year students enrolled in principles of biology at Â鶹ÉçÇø-Lock Haven recently participated in a hands-on laboratory experience using real-time Polymerase Chain Reaction (PCR) technology, a powerful molecular tool widely used in modern science.

Drs. Kyle Root (chemistry) and Barrie Overton (biology), at the start of the CU integration, negotiated with Bio-Rad Laboratories to get a real-time PCR machine at a discounted rate. The CU biology department, through an equipment ranking process, purchased the instrument and the chemistry department purchased the computer for the instrument.

Real-time PCR can be used to detect pathogens like COVID or RSV, to identify environmental DNA by ecology majors and for gene expression analysis in biochemistry.

Root and Overton recruited chemistry major Ray Hartman, of Linden, to work on a variety of independent studies to learn the instrument. Hartman is a senior and plans to graduate in May. Root and Overton felt it was important for Hartman to pass on his knowledge and experience using this sophisticated instrument to others in classes or who may be conducting research.

Hartman worked with Overton to develop a real-time PCR lab that could be used by first-year students. He also attended a biology lab and helped teach students how to use the instrument and understand the data.

Students responded positively to the experience, finding it helpful to learn from another student who had experience with the instrument - engaged in both the pre-lab and the hands-on portion of the lab.

Hartman continues to work with students in both the chemistry and biology programs, helping with research projects that include gene expression and pathogen detection. He also helped develop a student-friendly protocol that will be used in future classes and student research projects.

Root and Overton believe this experience highlights the strength of the CU-Lock Haven programs and the collaboration between departments and students across all CU campuses.

"This experience demonstrates CU-Lock Haven's commitment to hands-on learning and preparing students for future scientific careers," Overton said.

Students interested in ecology, biochemistry or biomedical sciences have the opportunity to work with modern molecular tools like real-time PCR early in their academic careers at Â鶹ÉçÇø.